Hemagglutination inhibition assay procedure

Ha and hi apply the process of hemagglutination, in which sialic acid receptors on the surface of red blood cells bind to the hemagglutinin glycoprotein found on the surface of influenza virus and create a network, or lattice structure, of. Qualification of the hemagglutination inhibition assay in support of. Human monoclonal antibodies generated from single human b cells were. Hemagglutination inhibition assay the hemagglutination inhibition hi assay is used to titrate the antibody response to a viral infection. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The hai assay detects serum antibodies to the viral hemagglutinin by measuring the inhibition of virusmediated agglutination of erythrocytes. A sensitive immunoassay procedure for the detection of the thrombopoieticstimulating factor tsf has been developed. The basis of the hai assay is that antibodies to that particular virus for example. This process is known as hemagglutination, and it is the basis of this assay. Hemagglutination inhibition tests are simple, sensitive, inexpensive, and rapid and therefore are often the method of choice for assaying antibodies to influenza a virus.

This video belongs to the section entitled serological tests that is part of the dvd avian influenza sampling procedures and laboratory testing funded by fao and the istituto zooprofilattico. Standardization of the hemagglutination inhibition hai assay for. Download citation hemagglutination inhibition assay the hemagglutination assay is a simple and easy method to obtain semiquantitative data on the sugar binding and specificity of a lectin. Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. This video belongs to the section entitled serological tests that is part of the dvd avian influenza sampling procedures and laboratory. Hemagglutinationinhibition assay for influenza virus subtype. The haemagglutinationinhibition hai assay is a traditional method for assessing immune responses to influenza virus haemagglutinin ha and for identifying influenza virus field isolates. The hi assay takes advantage of some viruses ability to hemagglutinate bind red blood cells, therefore forming a lattice and preventing the red blood cells from clumping. The principle behind the hemagglutination test is that the nucleic acids of viruses encode proteins, such as hemagglutinin, that are expressed on the surface of the. The national center for biomedical ontology was founded as one of the national centers for biomedical computing, supported by the nhgri, the nhlbi, and the nih common fund under grant u54hg004028. Pdf the hemagglutinininhibition assay for thrombopoietin.

Laboratory procedures serological detection of avian. Hemagglutinationinhibition hi assay is a classical laboratory procedure for the classification or subtyping of hemagglutinating viruses. The hemagglutination assay or haemagglutination assay ha and the hemagglutination inhibition assay hi. A viral suspension may agglutinate the red blood cells, thus preventing them from settling out of suspension.

The hemagglutination assay or haemagglutination assay and the hemagglutination inhibition assay were developed in 194142 by american virologist george hirst as methods for quantifying the relative concentration of viruses, bacteria, or antibodies. A general procedure for ha is as follows, a serial dilution of virus is prepared across the rows in a u or v bottom shaped 96well. Hemagglutination inhibition test an overview sciencedirect topics. Hemagglutination and hemagglutination inhibition test. Hemagglutination inhibition an overview sciencedirect. Standardization of hemagglutination inhibition assay for influenza. Therefore hemagglutination is inhibited when antibodies are present. Hemagglutination ha assay protocol the hemagglutination assay is a method for titering influenza viruses based on their ability to attach to molecules present on the surface of red blood cells. The haemagglutinationinhibition hai assay is a traditional method for assessing immune responses to influenza virus haemagglutinin ha and. An active lectin binds erythrocytes rbcs by recognising a cellsurface carbohydrate, forming a crosslinked network in suspension.